学术论文

      Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

      Using real-time reverse transcription PCR ten housekeeping genes from different abundance and functional classes in various human tissues were evaluated. The conventional use of a single gene for normalization leads to relatively large errors in a significant proportion of samples tested.
      作者: Vandesompele, Jo De Preter, Katleen Pattyn, Filip Poppe, Bruce Van Roy, Nadine De Paepe, Anne Speleman, Frank
      年,卷(期): 2002,
      关键词: Research