学术论文

      PEG胁迫下4个黑麦草品种生理生化指标响应与比较研究

      A study on the response and on the comparison of physiological and biochemical indexes of four Lolium perenne varieties under PEG stress

      摘要:
      利用不同浓度PEG-6000溶液模拟干旱胁迫,比较研究4个多年生黑麦草品种叶片生理生化指标响应.结果表明,不同PEG胁迫处理下,品种"欧必克"叶片相对含水量均最高且降幅最小,叶片电导率均最低,MDA含量增幅较小,叶片细胞膜系统有较强的忍耐干旱的能力;而品种"凤凰"忍耐干旱的性能相对较弱.胁迫下各品种叶片游离脯氨酸含量显著增加,随渗透胁迫加剧,品种"欧必克"脯氨酸含量急剧上升.低浓度胁迫下(5%PEG),各品种叶片SOD活性均提高,胁迫加剧时SOD活性则下降.PEG渗透胁迫导致4个多年生黑麦草品种叶片相对含水量下降、细胞膜透性增大、MDA含量增加、脯氨酸含量上升而SOD活性下降,但4个品种对应指标的变化幅度存在差异,品种"欧必克"的抗旱性最强,而"凤凰"的抗旱性最弱.
      Abstract:
      Response of physiological and biochemical indexes in leaves of four Lolium perenne varieties was compared under simulated drought stress using different concentrations of PEG solution. "Oupec", with the highest relative water content and least decreasing changes, the lowest electrical conductivities,and the smallest increasing rates of MDA contents under PEG stress had more tolerant membrane systems and was the most resistant to osmotic stress, while the resistance of "Polim" was relatively weaker. Proline accumulation in leaves of the four varieties increased and "Oupec" with rapid increases in proline contents had the strongest resistance to PEG stress. Activities of superoxide dismutase (SOD) in leaves of the four varieties increased during the initial stress stages (5% PEG) and decreased continuously with more severe stress. "Oupec" and "Polim" had the strongest and the weakest drought resistance respectively. The four L. perenne varieties had reduced relative water contents and activities of superoxide dismutase (SOD), increased permeability of plasma, MDA contents and proline accumulation under PEG stress and had differences in rates of change for the response indices.
      作者: 万里强 [1] 李向林 [1] 石永红 [2] 何峰 [1] 贾亚雄 [1]
      Author: WAN Li-qiang [1] LI Xiang-lin [1] SHI Yong-hong [2] HE Feng [1] JIA Ya-xiong [1]
      作者单位: 中国农业科学院北京畜牧兽医研究所,北京,100193 山西省农业科学院畜牧兽医研究所,山西,太原030032
      刊 名: 草业学报 ISTICPKU
      年,卷(期): 2010, 19(1)
      分类号: S543~+.601 Q945.78
      机标分类号: S51 X17
      在线出版日期: 2010年4月26日
      基金项目: 中国农业科学院北京畜牧兽医研究所基本科研业务费专项基金创新团队项目,国家科技支撑计划,引进国际先进农业科技计划(948计划)