以1,8-萘酰亚胺、壳聚糖、盐酸吗啉胍为原料,通过酰亚胺化、席夫碱等反应,设计合成了一种萘酰亚胺接枝壳聚糖类增强型荧光探针(NCFP).采用红外、核磁共振等技术对其结构进行表征,利用荧光光谱研究了其光谱性能及对金属离子的识别作用.研究结果表明,合成的荧光探针对Al3+具有较高的选择识别性能,且受常见离子的干扰较小.探针的荧光强度与Al3+浓度(29.90~253.41 μmol/L)有良好的线性关系,线性相关系数R2=0.9979,Al3+的最低检出限达4.279 μmol/L,Job's曲线表明,荧光探针与Al3+的络合比为1:1.
A fluorescent enhancement probe(for short:NCFP) was designed and synthesized based on the naphthalene imide derivatives grafted chitosan through imidization,schiff base reaction, and so on by using 1,8-naphthalene anhydride, chitosan, moroxydine hydrochloride as raw materials. The structure of the fluorescent probe was characterized by 1H NMR, FI-IR method, the spectral properties and identification of metal ions were studied with fluorescence spectroscopy. The results indicate that the fluorescent probe has strong fluorescence responses to Al3+, and remarkably high selectivity to Al3+than other metal ions. When the concentration of Al3+is in the range of 29.90-253.41 μmol/L, there is a good linearity between the fluorescence intensity of the probe and the concentration of Al3+. Linear correlation coefficient R2=0.997 9,the detection limit of the probe is 4.279 μmol/L to Al3+. The complexation ratio of probe/Al3+is 1:1 from the method of Job's plot by fluorescence pectrophotometry.