学术论文

      自组装短肽R2I4R2对皮肤创伤快速修复过程的研究

      Self-assembling Peptide R2I4R2 for Skin Wounds Repairing

      摘要:
      研究探索自组装短肽R2I4R2在人皮肤成纤维细胞体外三维培养的应用效果与对创伤修复过程的作用.通过圆二色谱仪分析不同时间、温度和离子条件对其二级结构的影响;刚果红染色宏观检测短肽自组装情况;体外培养人皮肤成纤维细胞探索细胞在R2I4R2形成的纳米纤维网络中的生长状态及凋亡情况;建立SD大鼠皮肤创伤模型,HE染色与免疫组织化学检测其对皮肤创伤修复的病理变化.结果表明,R2I4R2在不同条件下均可形成较为稳定的二级结构;自组装24h后可形成均一稳定的膜片状结构,为细胞三维培养提供支架;人皮肤成纤维细胞可在R2I4R2形成的纳米纤维网络三维环境中生长且状态良好;动物实验表明,短肽R2I4R2可减少炎症、促进新生血管生成、加速皮肤创伤修复过程.自组装短肽R2I4R2作为新的纳米支架材料,可用于细胞三维培养与皮肤创伤修复.
      Abstract:
      The aim is proposed to understand the application of self-assembling peptide R2I4R2 3D cell culture human skin fibroblast in vitro and the role of the wound healing process.Circular dichroism was used to analysis its secondary structure influenced by different time,temperature and ion environment;Congo red staining was used to obtain the macro change of R2I4R2 hydrogel;R2I4R2 was used to 3D cell culture material to get the growth state of human skin fibroblast cell strain;AO / EB staining was used to study cell apoptosis in 3 D culture environment;SD rat skin wound healing model was founded to check pathological changes by HE staining and immunohistochemical staining.These results indicated that R2I4R2 can form a stable secondary structure in some conditions;24 hours after self-assembling,R2I4R2 can form a homogeneous and stable membrane-like structure for 3D cell culture;Human skin fibroblasts had a hearty growing situation in hydrogel;The data in animal experiments show that R2I4R2 can reduce inflammation,promote neovascularization,accelerate the skin wound repair process.It demonstrate this short peptide can be used to a new material in 3D cell culture and skin trauma repairing and beyond.
      Author: ZHANG Hui-nan LI Meng-meng WEN Jing WU Shu-yi LAN Shi-jian LUO Zhong-li
      作者单位: 重庆医科大学基础医学院分子医学与肿瘤研究中心 重庆400016
      刊 名: 中国生物工程杂志 ISTICPKU
      Journal: China Biotechnology
      年,卷(期): 2018, 38(2)
      分类号: R318.08
      在线出版日期: 2018年5月17日
      基金项目: 国家自然科学基金,重庆市基础与前沿研究(重点)CQ (CSTC),感谢重庆医科大学科研培训基金,重庆医科大学基础医学院苗圃基金(JC201514)对本研究的支持与资助