学术论文

      氮素水平对不同品种茶树光合及叶绿素荧光特性的影响

      Characteristics of Photosynthetic and Chlorophyll Fluorescence of Tea Varieties under Different Nitrogen Application Levels

      摘要:
      为探明氮素水平对不同品种茶树的光合系统的影响机制,以'福鼎大白茶'、'保靖黄金茶1号'、'白毫早'两年生茶苗为材料,设置不施氮N0(0 g)、低氮N1(11 g)、中氮N2(22 g)和高氮N3(33 g)4个氮素[(NH4)2SO4]水平的盆栽实验,研究了铵态氮对3个品种茶树的生长势、叶片叶绿素含量、光合参数与叶绿素荧光参数的影响.结果表明:(1)施氮处理能够显著促进茶树的生长,提高茶树叶片叶绿素含量、净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr) ,降低胞间CO2浓度(Ci) ,并以N2处理最好,但水分利用率(WUE)在3个品种茶树间表现不同. (2)在N2处理下,3个茶树品种的叶片光系统Ⅱ(PSⅡ)暗适应下的最大光化学效率(Fv/Fm) 、光化学猝灭系数(qP) 、PSⅡ的相对电子传递速率(rETR)亦增加最大,非光化学淬灭系数(NPQ)降低. (3)茶树叶片叶绿素含量与光合参数间存在着一定的联系,并且具有品种特异性.研究发现,适量施氮能够显著增加茶树叶绿素含量、气孔导度、光合活性,从而使得各品种茶树净光合速率增加;氮素水平对各茶树品种的光合及荧光特性影响存在差异,水分利用率亦具有品种特异性;生产中应综合叶绿素含量、光合作用参数、叶绿素荧光参数,可快速、直观地评价不同品种茶树对氮素营养的内在需求,为茶园施肥管理提供指导.
      Abstract:
      In order to ascertain the mechanism of nitrogen levels on the photosynthetic system in different tea varieties ,we investigated the grow th vigor ,chlorophyll content ,photosynthetic rate and chlorophyll fluorescence parameters in three tea varieties (Fuding dabai tea ,Baojing huangjin tea No.1 and Baihaozao ) by the pot experiment under nitrogen stress [(control :N0(0 g) ,low nitrogen :N1(11 g) ,suitable nitro-gen :N2(22) ,high nitrogen :N3(33 g)].Results showed that :(1) Nitrogen application significantly pro-moted tea grow th ,and the chlorophyll content ,net photosynthetic rate (Pn) ,stomatal conductance (Gs) , transpiration rate (Tr) increased after nitrogen application ,while the intercellular CO2concentration (Ci) decreased ,of which N2treatment had the greatest impact to them.The variation trends of water use effi- ciency (WUE) in 3 tea varieties were different with each other.(2)After treated with N2,the primary maximum photochemical efficiency of PSⅡ(Fv/Fm) ,photochemical quenching coefficient (qP) and the rel-ative electron transport rate of PS Ⅱ(rETR) also increased most in three varieties ,while the non-photo-chemical quenching coefficient (N PQ) decreased.(3) The results indicated that there was a correlation be-tween the chlorophyll content and photosynthetic parameters in tea leaves ,and it represented a variety specificity between them.In conclusion ,nitrogen application could increase the chlorophyll content ,Gs and qP ,and enhance net photosynthetic rate.Compared with control ,the photosynthetic characteristics , chlorophyll fluorescence parameters and W UE showed different characteristics in tea varieties under differ-ent nitrogen levels.It indicates that we can rapidly evaluate the needs of nitrogen nutrition in tea plants based on the chlorophyll content ,photosynthetic characteristics and chlorophyll fluorescence parameters , and provide guidance for the management of tea plantation.
      作者: 向芬 [1] 李维 [1] 刘红艳 [1] 周凌云 [1] 江昌俊 [2]
      Author: XIANG Fen [1] LI Wei [1] LIU Hongyan [1] ZHOU Lingyun [1] JIANG Changjun [2]
      作者单位: 湖南省农业科学院 茶叶研究所,长沙,410125 安徽农业大学 茶树生物学与资源利用国家重点实验室,合肥,230036
      刊 名: 西北植物学报 ISTICPKU
      年,卷(期): 2018, 38(6)
      分类号: Q945.79
      在线出版日期: 2018年8月15日
      基金项目: 湖南省自然科学基金,安徽农业大学茶树生物学与资源利用国家重点实验室开放基金,湖南农业科技创新资金