学术论文

      美洲大蠊提取液对大鼠难愈合创面VEGF表达影响的研究

      On Effect of American Periplaneta Extract on VEGF’s Gene Expression of Rats’Chronic Refractory Wound

      摘要:
      目的:探讨外用单味动物药促进创面愈合的机制。方法:将50只大鼠分为急性创面组、模型组、贝复济组、美洲大蠊提取液组,用RT-PCR实验方法,观察大鼠皮肤修复组织VEGFmRNA的表达。结果:VEGFmRNA的表达在第5、7、11天,急性愈合创面组 VEGFmRNA 的表达量显著高于美洲大蠊提取液组、贝复济组及模型组(P<0.01);在第5、7、11天,美洲大蠊提取液组、贝复济组VEGFmRNA的表达量显著高于模型组(P<0.01);在第5、11天,美洲大蠊提取液组与贝复济组VEGFmRNA的表达量有统计学差异(P<0.05);第7天,肉芽组织生长期,美洲大蠊提取液组与贝复济组VEGFmRNA的表达量无统计学差异(P>0.05)。结论:美洲大蠊提取液通过提高VEGFmRNA的表达来促进创面愈合。
      Abstract:
      Objective to study the external use of traditional Chinese medicine of single animal to promote the mechanism of wound healing.Methods dividing 50 rats into acute wound group,model group,rb-bfgf group,and American periplaneta extract group,using RT-PCR testing method to observe the rat’ s expression of skin repairing tissue of VEGFmRNA.Results the expression of VEGFmRNA on the fifth day,the seventh day and the eleventh day,the acute healing wound group has a significantly higher a-mount of expression than American periplaneta extract group and rb-bfgf group (P<0. 01);On the fifth day,the seventh day and the eleventh day,the expressions of VEGFmRNA of both American periplaneta extract group and rb-bfgf group are significantly higher than model group (P<0. 01);On the fifth day and the eleventh day,American periplaneta extract group has difference with the rb-bfgf group on the expression quantity of VEGFmRNA (P<0. 05 ),on the seventh day,which is the growing period of granulation tis-sue,American periplaneta extract group has no difference with rb-bfgf group on the expression quantity of VEGFmRNA (P>0. 05 ). Conclusion American periplaneta extract promotes the wound healing by increasing the expression of VEGFmRNA.
      作者: 曾娟妮 [1] 刘筱 [2] 伍伟明 [2] 尹婷 [2] 吴志荣 [2] 李文华 [2]
      Author: ZENGJuanni [1] LIUXiao [2] WUWeiming [2] YINTing [2] WUZhirong [2] LIWenhua [2]
      作者单位: 湖南中医药大学第二附属医院,湖南长沙,410000 湖南中医药大学,湖南长沙,410208
      年,卷(期): 2016, 25(19)
      分类号: R285.5
      机标分类号: TQ4 S86
      在线出版日期: 2016年10月31日
      基金项目: 本课题受湖南省中医药科研计划项目(编号201466)支助;湖南省教育厅重点学科中医外科学一肛肠方向专项支助。