学术论文

      白藜芦醇对大鼠血液高凝态的抑制作用及其与NF-κB表达的关系

      Inhibitory effect of resveratrol on the hypercoagulable state in rats and its relationship with the expression of NF-κB

      摘要:
      目的 研究白藜芦醇对于大鼠血液高凝态形成的影响及其与NF-κB表达的关系.方法 SD大鼠分空白组、模型组、白藜芦醇高剂量组(60 mg/kg)、白藜芦醇低剂量组(30 mg/kg)和阿司匹林组(10 mg/kg),连续灌胃给药7 d,采用肾上腺素联合冰浴法和凝血酶法建立高凝态大鼠模型,采集血样测凝血酶原时间(prothrombin time,PT)、活化部分凝血活酶时间(activated partial thromboplastin time,APTT)及全血粘度;收集血管内皮细胞,采用蛋白印迹法测NF-κB表达情况.结果 空白组、白藜芦醇高剂量组和阿司匹林组较模型组PT、APTT显著延长(P<0.05),血液粘度显著降低(P<0.05);白藜芦醇高剂量组血管内皮细胞NF-κB表达较模型组明显降低(P<0.05).结论高剂量白藜芦醇(60 mg/kg)可抑制大鼠血液高凝态形成,这种作用可能与其降低NF-κB的表达有关.
      Abstract:
      Objective The topic of this study was to study the inhibitory effect of resveratrol on hypercoagulable state in rats and its relationship with the expression of NF-κB. Methods Rats were divided into control and model groups, and resveratrol ( high dose, 60 mg/kg ) , resveratrol ( low dose, 30 mg/kg ) and aspirin ( 10 mg/kg ) were given by intragastric administration for 7 d. Then, the rats were treated by adrenaline with ice bath or thrombin to generate hypercoagulable state. Blood samples were collected to test the prothrombin time (PT), activated partial thromboplastin time ( APTT) and blood viscosity. Vascular endothelial cells were collected to detect the expression of NF-κB by western blotting. Results PT and APTT values of rats in the control, resveratrol (high dose) and aspirin groups were significantly increased than the model group (P< 0. 05). Blood viscosity of rats in the control, resveratrol (high dose) and aspirin groups was significantly lower than the model group (P< 0. 05). The expression of NF-κB was decreased than that of the model group (P< 0. 05). Conclusions Resveratrol in high dose (60 mg/kg) can inhibit hypercoagulable state of rat, which may be related with its effect on decreasing the expression of NF-κB.
      作者: 王立文 [1] 沈晓洁 [1] 吴倩 [2]
      Author: WANG Li-wen [1] SHEN Xiao-jie [1] WU Qian [2]
      作者单位: 无锡卫生高等职业技术学校护理系,江苏 无锡,214028 中国药科大学药学院药理系,南京,210009
      刊 名: 中国比较医学杂志 ISTICPKU
      年,卷(期): 2017, 27(12)
      分类号: R-33
      在线出版日期: 2018年1月5日
      基金项目: 无锡市医院管理中心科研项目