学术论文

      左归丸对少弱精子症大鼠模型生殖内分泌激素的作用研究

      Effects of Zuogui pill on reproductive endocrine hormones in rats with oligoasthenospermia

      摘要:
      目的:观察左归丸对雷公藤多苷(GTW)诱导的少弱精子症大鼠模型的精子参数、睾丸/附睾系数、生殖激素水平、附睾肉毒碱含量的预防作用.方法:将36只雄性SD大鼠随机平均分为正常组、模型组及左归丸组,每组12只,共计8周.模型组前4周用去离子水进行干预,5~8周用40mg/(kg·d)的雷公藤多苷进行造模;左归丸治疗组前4周用6g/(kg·d)的左归丸混悬液进行预防性干预,5~8周用40mg/(kg·d)的雷公藤多苷进行干预.检测大鼠精子参数;测量睾丸的重量,计算睾丸脏器系数;测定血清黄体生成素(LH)、卵泡刺激素(FSH)、雌激素(E2)、泌乳素(PRL)、睾酮(T)、抑制素B(INHB)及附睾肉毒碱(EC)含量.结果:左归丸组与模型组相比,大鼠睾丸脏器系数、精子质量(活力、数量)、血清FSH、LH、T、IN-HB、EC含量方面的差异具有统计学意义(P<0.05或P<0.01);但INHB、EC水平仍与正常组有一定差异(P<0.05).结论:前期应用左归丸能够有效减少GTW对大鼠生殖器官的损伤程度,包括对一般状态、睾丸/附睾脏器指数、精子质量及生殖激素水平、附睾肉毒碱含量的影响,具有一定的预防作用.
      Abstract:
      Objectives:To observe the preventive effect of Zuogui Pill on sperm parameters,testis/epididymal coefficient,reproductive hormone level and carnitine content in epididymis of rats with oligoasthenospermia induced by tripterygium wilfordii (GTW).Methods:36 male Sprague-Dawley rats were randomly divided into normal group,model group and Zuogui pill group,with 12 rats in each group,for a total of 8 weeks.The rats in model group were treated with deionized water for the first 4 weeks,and then the rats were treated with 40rag / (kg · d) Tripterygium wilfordii for 5-8 weeks.The rats in Zuogui pill group were treated with 6g/ (kg · d) Zuogui pill for preventive intervention for the first 4 weeks,and 40mg / (kg · d) of the tripterygium glycosides to intervene for 5-8 weeks.(LH),follicle-stimulating hormone (FSH),estrogen (E2),prolactin (PRL) and testosterone (T)were measured,and the testis weight was measured.Inhibin B (INHB) and epididymal carnitine (EC) were measured.Results:The differences in contents of FSH,LH,T,INHB and EC in testis between Zuogui pill group and model group were significantly different (P < 0.05 or P < 0.01),and the levels of INHB and EC in Zuogui pill group and model group were still different from these of normal group (P < 0.05).Conclusion:Zuogui Pill can effectively reduce the damage degree of GTW on the reproductive organs of rats,including the effects of general state,testis/epididymis organ index,sperm quality and reproductive hormone levels,and the content of carnitine in epididymis.It is proved that Zuogui Pill has certain preventive effect on rats with oligoasthenospermia.
      Author: WANG Bin MA Jianxiong MA Huangfu ZHU Yutian WANG Jisheng DANG Jin DONG Lei YAO Zeyu LI Haisong
      作者单位: 北京中医药大学东直门医院男科,北京100700;北京中医药大学男科研究所,北京100700
      刊 名: 中国性科学 ISTIC
      年,卷(期): 2017, 26(11)
      分类号: R277.33
      在线出版日期: 2017年12月22日
      基金项目: 国家自然科学基金