目的探讨循环DNA在急性心肌梗死中的应用价值.方法采集40例急性心肌梗死患者发病后48 h内和恢复期静脉血各2 ml,采用双重荧光定量PCR技术检测血浆DNA水平,同时检测心肌酶谱和肌钙蛋白T(cTnT).同期采集40例健康体检者静脉血作为对照组.结果对照组和急性心肌梗死患者治疗前循环DNA水平分别为(21.5±10.7) ng/ml和(253.6±45.7) ng/ml,后者显著高于前者(P=0.000);急性心肌梗死患者血清心肌酶谱和cTnT水平均显著高于对照组(P值均<0.05);治疗后血浆循环DNA水平显著下降(P=0.000);心肌酶谱和cTnT水平均显著降低,与治疗前相比P值均<0.05;循环DNA水平与CK-MB 、cTnT水平呈显著正相关,r值分别为0.613和0.654,P值分别为0.032和0.021.结论循环DNA可作为一种反映心肌细胞损伤的灵敏检测指标.
Objective To explore clinical value of circular DNA in acute myocardial infarction. Methods Venous blood (2ml/head) of 40 healthy control and 40 patients with acute myocardial infarction within 48h of onset of il ness and convalescent period was col ected. The level of plasma circular DNA was detected by duplex real-time polymerase chain reaction assay. The levels of myocardial enzyme spectrum and cardiac troponin T ( cTnT) were detected by biochemistry method. Results The level of circular DNA in control group and group of acute myocardial infarction before treatment was (21.5±10.7)ng/ml and (253.6±45.7) ng/ml, respectively (P=0.000). The levels of serum myocardial enzyme spectrum and cTnT before treatment in patients with acute myocardial infarction were significantly higher than those of control group(P<0.05).The level of circular DNA after treatment in patients with acute myocardial infarction was significantly decreased compared with that before treatment (P=0.000), the levels of myocardial enzyme spectrum and cTnT were also significantly reduced (P<0.05). There was significant correlation between the level of circular DNA and those of CK-MB and cTnT, r=0.613, 0.654, P=0.032, 0.021. Conclusion Circular DNA can be used as a marker of sensitively reflecting myocardial cel injury.